Recombinant Human Pepsinogen A5/PGA5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8457-AS
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human Pepsinogen A5/PGA5 protein
Ile16-Ala388, with C-terminal 6-His tag
Accession # P0DJD9
Ile16-Ala388, with C-terminal 6-His tag
Accession # P0DJD9
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile16 & Val63
Predicted Molecular Mass
41 kDa
SDS-PAGE
39-51 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >15,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >15,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
8457-AS
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Pepsinogen A5/PGA5
Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 24 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.
References
- Athauda, S.B. et al. (1989) J. Biochem. 106:920.
- Kageyama, T. et al. (1989) J. Biochem. 105:15.
- Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
- Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
- Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
- Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
- Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.
Alternate Names
PGA5
Entrez Gene IDs
5222 (Human)
Gene Symbol
PGA5
UniProt
Additional Pepsinogen A5/PGA5 Products
Product Documents for Recombinant Human Pepsinogen A5/PGA5 Protein, CF
Product Specific Notices for Recombinant Human Pepsinogen A5/PGA5 Protein, CF
For research use only
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