Recombinant Human Pepsinogen C/PGC Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6186-AS

R&D Systems, part of Bio-Techne
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6186-AS-010
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Key Product Details

Source

NS0

Accession #

P20142

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Pepsinogen C/PGC Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Pepsinogen C/PGC protein
Met1-Ala388, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala17 & Leu43

Predicted Molecular Mass

41 kDa & 38 kDa

SDS-PAGE

35-40 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6186-AS
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Pepsinogen C/PGC

Pepsinogen C (progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is synthesized as an inactive zymogen that is converted into its active mature form at low pH by sequential cleavage of the prosegment in an autocatalytic process (1). Polymorphisms in this gene are associated with susceptibility to gastric cancers (2). Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis (3).

References

  1. Richter, C. et al. (1998) Biochem. J. 335:481.
  2. Liu, H. J. et al. (2003) World J. Gastroenterol. 9:50.
  3. Plebani, M. (1993) Crit. Rev. Clin. Lab. Sci. 30:273.

Alternate Names

Gastricsin, PEPC, PGC, PGII

UniProt

P20142

Additional Pepsinogen C/PGC Products

Product Documents for Recombinant Human Pepsinogen C/PGC Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Pepsinogen C/PGC Protein, CF

For research use only

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