Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11486-F2

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q15063.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Periostin/OSF-2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Periostin/OSF-2 protein
Asn22-Gln808 (C60A) with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn22

Predicted Molecular Mass

89 kDa

SDS-PAGE

68-90 kDa, under reducing conditions.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.500-6.00 μg/mL.

Scientific Data Images for Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF

Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein Bioactivity.

Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein (Catalog # 11486-F2) supports the adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.500-6.00 μg/mL.

Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein (Catalog # 11486-F2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-90 kDa, under reducing conditions.

Formulation, Preparation and Storage

11486-F2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% amino acid sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). Periostin is also up-regulated in many carcinomas (2, 8) and induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Further, Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References

  1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
  2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  7. Li, G. et al. (2006) Atherosclerosis 188:292.
  8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
  10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
  11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
  12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
  13. Snider, P. et al. (2008) Circ. Res. 102:752.
  14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
  15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
  16. Sehra, S. et al. (2011) J. Immunol. 186:4959.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Gene Symbol

POSTN

UniProt

Q15063.1

Additional Periostin/OSF-2 Products

Product Documents for Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF

For research use only

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