Recombinant Human Periostin/OSF-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3548-F2
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Periostin/OSF-2 protein
Asn22-Gln836, with a C-terminal 6-His tag
Asn22-Gln836, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn22
Predicted Molecular Mass
91.7 kDa
SDS-PAGE
85-90 kDa, reducing conditions
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
Formulation, Preparation and Storage
3548-F2
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Periostin/OSF-2
References
- Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
- Gillan, L. et al. (2002) Cancer Res. 62:5358.
- Takeshita, S. et al. (1993) Biochem. J. 294:271.
- Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
- Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
- Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
- Li, G. et al. (2006) Atherosclerosis 188:292.
- Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
- Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
- Erkan, M. et al. (2007) Gastroenterology 132:1447.
- Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
- Kudo, Y. et al. (2012) PLoS One 7:e44488.
- Snider, P. et al. (2008) Circ. Res. 102:752.
- Kuhn, B. et al. (2007) Nat. Med. 13:962.
- Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
- Sehra, S. et al. (2011) J. Immunol. 186:4959.
Long Name
Osteoblast Specific Factor 2
Alternate Names
Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52
Gene Symbol
POSTN
UniProt
Additional Periostin/OSF-2 Products
Product Documents for Recombinant Human Periostin/OSF-2 Protein, CF
Product Specific Notices for Recombinant Human Periostin/OSF-2 Protein, CF
For research use only
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