Recombinant Human PGLYRP1/PGRP-S Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2590-PGB

R&D Systems, part of Bio-Techne
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2590-PGB-050
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Key Product Details

Source

NS0

Accession #

O75594

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PGLYRP1/PGRP-S Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PGLYRP1/PGRP-S protein
Gln22-Pro196, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln22 predicted

Predicted Molecular Mass

20 kDa

SDS-PAGE

23-27 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When peptidoglycan is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PGLYRP1/PGRP-S that produces 50% optimal binding response is 0.75-4.5 ng/mL.

Scientific Data Images for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Bioactivity graph of recombinant human PGLYRP1 / PGRP-S Protein

Bioactivity of Recombinant Human PGLYRP1/PGRP-S protein

When peptidoglycan is coated at 1 µg/mL, 100 µL/well, Recombinant Human PGLYRP1/PGRP-S binds with an ED50 of 0.75-4.5 ng/mL.

Formulation, Preparation and Storage

2590-PGB
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP1/PGRP-S

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71% and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

References

  1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
  2. Steiner, H. (2004) Immunol. Rev. 198:83.
  3. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
  5. Dziarski, R. et al. (2003) Blood 102:689.

Long Name

Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1

Alternate Names

PGRP-S, PGRPS, TAG7, TNFSF3L

Entrez Gene IDs

8993 (Human); 21946 (Mouse)

Gene Symbol

PGLYRP1

UniProt

O75594

Additional PGLYRP1/PGRP-S Products

Product Documents for Recombinant Human PGLYRP1/PGRP-S Protein, CF

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Product Specific Notices for Recombinant Human PGLYRP1/PGRP-S Protein, CF

For research use only

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