Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9828

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9828-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q9UKJ0.1

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all PILR-beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PILR-beta protein
Human PILR- beta 
(Gln20-Ala189)
Accession # Q9UKJ0.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20, as confirmed by mass spectrometry.

Predicted Molecular Mass

48 kDa

SDS-PAGE

55-68 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human PILR‑ beta Fc Chimera Avi-tag binds with an ED50 of 0.600-6.00 μg/mL.

Scientific Data Images for Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF

When Recombinant Human CL-P1/COLEC12 (2690-CL) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human PILR‑ beta Fc Chimera Avi-tag (Catalog # AVI9828) binds with an ED50 of 0.600-6.00 μg/mL.

Recombinant Human PILR-beta Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human PILR-beta Fc Chimera Avi-tag Protein (Catalog # AVI9828) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-68 kDa.

Formulation, Preparation and Storage

AVI9828
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PILR-beta

Paired immunoglobulin-like type 2 receptor beta (PILR-beta) is a type I transmembrane (TM) glycoprotein that belongs to the Ig superfamily (1). PILR-beta is the activating counterpart to the inhibitory immunoreceptor PILR-alpha, forming a set of paired immune regulatory receptors responsible for regulating inflammatory responses (1). Mature human PILR-beta contains a V-type Ig-like extracellular domain (ECD) with a siglec-like fold, a single transmembrane domain, and a truncated cytoplasmic tail (2, 3). The transmembrane domain of PILR-beta contains a positively charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% aa sequence identity with the ECD of mouse PILR-beta. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, such as microglial cells (2,4). It is a binding partner for DAP12 and CD99, and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4,5). Our Avi-tag Biotinylated PILR-beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
  2. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
  3. Lu, Q. et al. (2014) PNAS 111:8221.
  4. Tato, C.M. et al. (2012) PLoS One 7:e31690.
  5. Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
  6. Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
  7. Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467

Long Name

Paired-Ig-likeType 2 Receptor beta

Alternate Names

FDFACT1, FDFACT2, PILRB, PILRbeta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Gene Symbol

PILRB

UniProt

Q9UKJ0.1

Additional PILR-beta Products

Product Documents for Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF

For research use only

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