Recombinant Human PILR-beta His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10319-PR

R&D Systems, part of Bio-Techne
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10319-PR-050
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Key Product Details

Source

HEK293

Accession #

Q9UKJ0.1

Conjugate

Unconjugated

Applications

Bioactivity

View all PILR-beta Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PILR-beta protein
Gln20-Ala189, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20, as confirmed by mass spectrometry

Predicted Molecular Mass

20 kDa

SDS-PAGE

30-40 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CL-P1/COLEC12  (Catalog # 2690-CL) is immobilized at 2 μg/mL (100 μL/well), it binds Recombinant Human PILR-beta (Catalog # 10319-PR) with an ED50 of 1-10 μg/mL

Scientific Data Images for Recombinant Human PILR-beta His-tag Protein, CF

2 μg/lane of Recombinant Human PILR-beta His-tag Protein (Catalog # 10319-PR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 30-40 kDa under reducing and non-reducing conditions.

Formulation, Preparation and Storage

10319-PR
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PILR-beta

Paired immunoglobulin-like type 2 receptor beta (PILR-beta) is a type I transmembrane (TM) glycoprotein that belongs to the Ig superfamily (1). PILR-beta is the activating counterpart to the inhibitory receptor immunoreceptor PILR-alpha which contains a tyrosine-based inhibitory motif (ITIM) (1). Mature human PILR-beta contains a V-type Ig-like extracellular domain (ECD) with a siglec-like fold, a single TM domain, and a truncated cytoplasmic tail (2, 3). The TM domain of PILR-beta contains a positively-charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% amino acid sequence identity with its mouse counterpart. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, including microglial cells (2, 4). It is a binding partner for DAP12 and CD99 and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4, 5).

References

  1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
  2. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
  3. Lu, Q. et al. (2014) PNAS 111:8221.
  4. Tato, C.M. et al. (2012) PLoS One 7:e31690.
  5. Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
  6. Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
  7. Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467.

Long Name

Paired-Ig-likeType 2 Receptor beta

Alternate Names

FDFACT1, FDFACT2, PILRB, PILRbeta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Gene Symbol

PILRB

UniProt

Q9UKJ0.1

Additional PILR-beta Products

Product Documents for Recombinant Human PILR-beta His-tag Protein, CF

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Product Specific Notices for Recombinant Human PILR-beta His-tag Protein, CF

For research use only

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