Recombinant Human Plexin A3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10519-PA

R&D Systems, part of Bio-Techne
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10519-PA-050
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Key Product Details

Source

CHO

Accession #

NP_059984.3

Conjugate

Unconjugated

View all Plexin A3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Plexin A3 protein
Asn20-Ala1220, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

133 kDa

SDS-PAGE

135-160 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human Plexin A3 His-tag Protein, CF

Recombinant Human Plexin A3 His-tag Protein SDS-PAGE

Recombinant Human Plexin A3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Plexin A3 His-tag Protein (10519-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-160 kDa.

Formulation, Preparation and Storage

10519-PA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A3

Plexin A3, also known as Plexin-4, is a member of the large Plexin family of conserved transmembrane receptors that mediate Semaphorin functions (1, 2). There are four classes plexins (A, B, C, and D) and they share a similar domain structure: An extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and an approximately 600 residue-long cytoplasmic region (2). The ECD of mature, human plexinA-3 consists of a Sema domain and four IPT (Ig-like, plexins, transcription factors) domains and shares 93% amino acid identity with both mouse and rat PlexinA-3. Plexin A3 is a coreceptor for class 3 semaphorins and plays an important role in axon guidance in the developing nervous system and regulates the migration of sympathetic neurons (3, 4). Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin (4). Plexin A3 is required for normal dendrite spine morphology in pyramidal neurons and may play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system (5). The cytoplasmic domain of plexinA-3 is required for the activation of down-stream signaling events in the cytoplasm (6).

References

  1. Pascoe, H.G. et al. (2015) Prog Biophys Mol Biol. 118(3):161.
  2. Tamagnone L et al. (1999) Cell. 99:71.
  3. Alto, L. T. et al. (2017) Methods Mol Biol. 1493:1.
  4. Yaron, A. et al. (2005) Neuron. 45:513.
  5. Cheng, H. J. et al. (2001) Neuron. 32:249.
  6. He H. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:15610.

Alternate Names

Plxn3, PLXN4, PLXNA3, SEX, XAP-6

Entrez Gene IDs

55558 (Human); 18846 (Mouse); 309280 (Rat)

Gene Symbol

PLXNA3

UniProt

NP_059984.3

Additional Plexin A3 Products

Product Documents for Recombinant Human Plexin A3 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Plexin A3 His-tag Protein, CF

For research use only

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