Recombinant Human PNMT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7854-MT

R&D Systems, part of Bio-Techne
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7854-MT-020
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Key Product Details

Source

E. coli

Accession #

P11086

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PNMT Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PNMT protein
Met1-Leu282, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

32 kDa

SDS-PAGE

29-31 kDa, reducing conditions

Activity

Measured by its S-Adenosyl-L-Methionine (SAM)-dependent ability to transfer a methyl group to norepinephrine.
The specific activity is >2.5 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7854-MT
Formulation Supplied as a 0.2 μm filtered solution in Tris, EDTA, Brij-35 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PNMT

Phenylethanolamine N-methyltransferase (PNMT) is the enzyme that catalyzes biosynthesis of epinephrine from norepinephrine using S-Adenosyl-L-Methionine (SAM) as the methyl donor (1). The enzyme is expressed in high levels in the adrenal medulla, where epinephrine is synthesized (2). It is also responsible for synthesis of epinephrine in the central nervous system as peripheral epinephrine does not cross the blood-brain barrier (3, 4). The function of PNMT has been implicated in regulation of blood pressure, respiration, body temperature, and in neurodegeneration observed in Parkinson’s disease and Alzheimer’s disease (5-7).

References

  1. Kirshner, N. et al. (1957) Biochim. Biophys. Acta. 24:658.
  2. Axelrod, J. (1962) J. Biol. Chem. 237:1657.
  3. Weil-Malherbe, H. et al. (1959) Science 129:1226.
  4. Ciaranello, R. D. et al. (1969) Nature 221:368.
  5. Saavedra, J. M. et al. (1976) Science 191:483.
  6. Rothballer, A. B. (1959) Pharmacol. Rev. 11:494.
  7. Mann, M. B. (2001) Am. J. Med. Genet. 105:312.

Long Name

Phenylethanolamine N-Methyltransferase

Alternate Names

Noradrenaline N-methyltransferase, PENT, PNMTase

Entrez Gene IDs

5409 (Human); 18948 (Mouse)

Gene Symbol

PNMT

UniProt

P11086

Additional PNMT Products

Product Documents for Recombinant Human PNMT Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PNMT Protein, CF

For research use only

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