Recombinant Human Podocin/NPHS2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9287-PO

R&D Systems, part of Bio-Techne
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9287-PO-050
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Key Product Details

Source

E. coli

Accession #

Q9NP85

Conjugate

Unconjugated

Applications

Bioactivity

View all Podocin/NPHS2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Podocin/NPHS2 protein
Lys259-Leu383, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant human Nephrin is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Podocin/NPHS2 binds with a typical ED50 of 0.8-4 μg/mL.

Formulation, Preparation and Storage

9287-PO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocin/NPHS2

Podocin, encoded by the NPHS2 gene, is an approximately 50 kDa membrane protein that plays an important role in podocyte function in the kidney. Loss of Podocin function results in albuminuria, hypercholesterolemia, hypertension, and renal failure (1). Human Podocin consists of a 102 amino acid (aa) cytoplasmic domain, a 21 aa intramembrane segment, and a second 262 aa cytoplasmic domain (2, 3). Alternative splicing generates a short isoform with a 68 aa deletion in the second cytoplasmic domain (4, 5). Within aa 259-383 (the region common to both isoforms), human Podocin shares 90% aa sequence identity with mouse and rat Podocin. Podicin localizes to areas of cell-cell contact between podocytes in the renal glomerulus (2, 6, 7). It associates into oligomers and forms complexes with Nephrin, CAR, ZO-1, and the cation ion channel TRPC6 (6-9). It contributes to podocyte function by regulating the activation of TRPC6 and Nephrin mediated signaling (8, 9). Multiple polymorphisms in NPHS2 are associated with steroid-resistant nephrotic syndrome (2).

References

  1. Mollet, G. et al. (2009) J. Am. Soc. Nephrol. 20:2181.
  2. Boute, N. et al. (2000) Nat. Genet. 24:349.
  3. Roselli, S. et al. (2002) Am. J. Pathol. 160:131.
  4. Volker, L.A. et al. (2013) BMC Nephrol. 14:102.
  5. Relle, M. et al. (2011) Mod. Pathol. 24:1101.
  6. Shono, A. et al. (2007) J. Am. Soc. Nephrol. 18:2525.
  7. Schwarz, K. et al. (2001) J. Clin. Invest. 108:1621.
  8. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  9. Anderson, M. et al. (2013) Am. J. Physiol. Cell Physiol. 305:C276.

Alternate Names

Nephrosis 2, NPHS2, PDCN, SRN1

Entrez Gene IDs

7827 (Human); 170484 (Mouse); 170672 (Rat)

Gene Symbol

NPHS2

UniProt

Q9NP85

Additional Podocin/NPHS2 Products

Product Documents for Recombinant Human Podocin/NPHS2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Podocin/NPHS2 Protein, CF

For research use only

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