Recombinant Human Proprotein Convertase 2/PCSK2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6018-SE

R&D Systems, part of Bio-Techne
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6018-SE-010
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Key Product Details

Source

CHO

Accession #

P16519

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Proprotein Convertase 2/PCSK2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Proprotein Convertase 2/PCSK2 protein
Gly110-Asn638 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly110

Predicted Molecular Mass

59 kDa

SDS-PAGE

66-82 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (Catalog # ES013).
The specific activity is >180 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6018-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. 

References

  1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
  2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
  3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
  4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
  5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Long Name

Proprotein Convertase Subtilisin/Kexin Type 2

Alternate Names

NEC2, PC2, PCSK2, SPC2

Entrez Gene IDs

5126 (Human); 18549 (Mouse); 25121 (Rat)

Gene Symbol

PCSK2

UniProt

P16519 (Human)

Additional Proprotein Convertase 2/PCSK2 Products

Product Documents for Recombinant Human Proprotein Convertase 2/PCSK2 Protein, CF

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Product Specific Notices for Recombinant Human Proprotein Convertase 2/PCSK2 Protein, CF

For research use only

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