Recombinant Human Prostatic Acid Phosphatase/ACPP, CF

R&D Systems, part of Bio-Techne | Catalog # 6240-AP

R&D Systems, part of Bio-Techne
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6240-AP-010
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Key Product Details

Source

NS0

Accession #

P15309

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Prostatic Acid Phosphatase/ACPP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Prostatic Acid Phosphatase/ACPP protein
Met1-Gln379, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys33

Predicted Molecular Mass

41 kDa

SDS-PAGE

43-55 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >100,000 pmol/min/ug, as measured under the described conditions.

Formulation, Preparation and Storage

6240-AP
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: Prostatic Acid Phosphatase/ACPP

Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins (1). The activity optimum of ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists (2). The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer (3). Cellular ACPP has been shown to be a protein tyrosine phosphatase (4). Protein substrates include the epidermal growth factor receptor and HER-2 (5). Cellular ACPP is considered to function as a tumor suppressor (5).

References

  1. Apostol, I. et al. (1985) Acta. Biochim. Pol. 32:187.
  2. Schroeder, B. et al. (2007) Traffic 8:1676.
  3. Gutman, E.B. et al. (1936) Am. J. Cancer 28:485.
  4. Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351.
  5. Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805.

Alternate Names

ACP3, ACPP

Entrez Gene IDs

55 (Human); 563181 (Mouse); 56780 (Rat)

Gene Symbol

ACP3

UniProt

P15309 (Human)

Additional Prostatic Acid Phosphatase/ACPP Products

Product Documents for Recombinant Human Prostatic Acid Phosphatase/ACPP, CF

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Product Specific Notices for Recombinant Human Prostatic Acid Phosphatase/ACPP, CF

For research use only

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