Recombinant Human PSG6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9318-P6

R&D Systems, part of Bio-Techne
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9318-P6-050
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Key Product Details

Source

HEK293

Accession #

Q00889-2

Conjugate

Unconjugated

Applications

Bioactivity

View all PSG6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PSG6 protein
Gln35-His424, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36

Predicted Molecular Mass

45 kDa

SDS-PAGE

62-73 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.9-4.5 μg/mL.

Scientific Data Images for Recombinant Human PSG6 Protein, CF

Recombinant Human PSG6 Protein Binding Activity

Recombinant Human PSG6 Protein Binding Activity

Recombinant Human PSG6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.

Formulation, Preparation and Storage

9318-P6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSG6

Pregnancy-specific glycoprotein 6 (PSG6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).

References

  1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
  2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
  3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.
  4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
  5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
  6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.

Long Name

Pregnancy-specific beta-1-glycoprotein 6

Alternate Names

PSBG-10, PSBG-12, PSG-6, PSGGB

Entrez Gene IDs

5675 (Human)

Gene Symbol

PSG6

UniProt

Q00889-2

Additional PSG6 Products

Product Documents for Recombinant Human PSG6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSG6 Protein, CF

For research use only

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