Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3345-PS

R&D Systems, part of Bio-Techne
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3345-PS-050
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Key Product Details

Source

CHO

Accession #

AAC50061

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PSGL-1/CD162 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PSGL-1/CD162 protein
Human PSGL-1
(Gln42-Val295)
Accession # AAC50061		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln42

Predicted Molecular Mass

52.6 kDa (monomer)

SDS-PAGE

100-150 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
The ED50 for this effect is 0.04-0.32 µg/mL.

Reviewed Applications

Read 1 review rated 4 using 3345-PS in the following applications:

Scientific Data Images for Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

Recombinant Human PSGL-1/CD162 Fc Chimera Protein Bioactivity

Recombinant Human PSGL-1/CD162 Fc Chimera Protein Bioactivity

Recombinant Human PSGL-1/CD162 Fc Chimera (Catalog # 3345-PS) supports the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.04-0.32 μg/mL.

Formulation, Preparation and Storage

3345-PS
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSGL-1/CD162

PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Human PSGL-1 cDNA encodes 412 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (1). The ECD of human PSGL-1 contains 16 decameric repeats in the A allele (long form), which is expressed by 65-80% of the population. Alleles B and C contain 15 and 14 repeats, respectively, and mouse PSGL-1 contains 10-12 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Human PSGL-1 ECD shares less than 50% aa sequence identity with mouse, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-412) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2‑4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (LeX) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E-Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).

References

  1. Yang, J. et al. (1999) Thromb. Haemost. 81:1.
  2. Sako, D. et al. (1993) Cell 75:1179.
  3. Carlow, D.A. et al. (2009) Immunol. Rev. 230:75.
  4. Zarbock, A. et al. (2011) Blood 278:37.
  5. Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
  6. Sako, D. et al. (1995) Cell 83:323.
  7. Xia, L. et al. (2003) Blood 101:552.
  8. Gardiner, E.E. et al. (2001) Blood 98:1440.
  9. Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
  10. Veerman, K.M. et al. (2007) Nat. Immunol. 8:532.
  11. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  12. del Conde, I. et al. (2005) Blood 106:1604.
  13. Thomas, G.M. et al. (2009) J Exp. Med. 206:1913.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Entrez Gene IDs

6404 (Human); 20345 (Mouse); 363930 (Rat)

Gene Symbol

SELPLG

UniProt

AAC50061

Additional PSGL-1/CD162 Products

Product Documents for Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

Product Datasheets

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