Recombinant Human PSMA/FOLH1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4234-ZN
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human PSMA/FOLH1/NAALADase I protein
Lys44-Ala750, with an N-terminal 6-His tag
Lys44-Ala750, with an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
80 kDa
SDS-PAGE
110 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
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Formulation, Preparation and Storage
4234-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
References
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
- Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Alternate Names
FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I
Gene Symbol
FOLH1
UniProt
Additional PSMA/FOLH1/NAALADase I Products
Product Documents for Recombinant Human PSMA/FOLH1 Protein, CF
Product Specific Notices for Recombinant Human PSMA/FOLH1 Protein, CF
For research use only
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