Recombinant Human Pyridoxal Kinase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8658-PK

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8658-PK-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

O00764

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Pyridoxal Kinase/PDXK Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Pyridoxal Kinase/PDXK protein
Glu2-Leu312, with N-terminal Met and 6-His tag
Accession # O00764

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate Pyridoxal Hydrochloride.
The specific activity is >85 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8658-PK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Pyridoxal Kinase/PDXK

Pyridoxal Kinase (PDXK) phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate (PLP), an important cofactor in intermediary metabolism (1). PLP is involved in many aspects of macronutrient metabolism, neurotransmitter synthesis, histamine synthesis, as well as hemoglobin synthesis, function, and gene expression (2). PLP generally serves as a co-enzyme for many reactions and can help facilitate decarboxylation, transamination, racemization, elimination, replacement and beta-group inter-conversion reactions. In particular, PLP also is needed for the enzymatic reaction governing the release of glucose from glycogen. Overall, there are over 160 PLP-dependent enzymes, corresponding to ~4% of all known enzymatic activities (3). The classic clinical syndrome for B6 deficiency is a seborrhoeic dermatitis-like eruption, atrophic glossitis with ulceration, angular cheilitis, conjunctivitis, intertrigo, accompanied by neurologic symptoms of somnolence, confusion, and neuropathy (4).The neurotoxic effects of ophylline and ginkgotoxin are caused by their inhibitory activity against human PDXK (3, 5). Genetic variations of PDXK are associated with Parkinson disease, since PLP is involved in the biosynthesis of dopamine, a neurotransmitter linked to the disease characteristics such as motor and movement disorders (6). PDXK is also a potential drug target in the African trypanosomiasis (7). PDXK is a cytoplasmic enzyme and probably acts as a homodimer in vivo (8). The enzymatic activity of recombinant human CHKB is measured using a phosphatase-coupled method (9).

References

  1. Hanna, M.C. et al. (1997) J. Biol. Chem. 272:10756.
  2. Percudani R. and A. Peracchi (2003). EMBO Rep. 4: 850.
  3. Gandhi, AK et al. (2012) PLoS ONE. 7:e40954.
  4. Lichtstein, H.C. et al. (1945) J. Biol. Chem. 161:311.
  5. Kastner, U. et al. (2007) FEBS J. 274:1036.
  6. Vilarino-Guell, C. et al. (2010) Ann. Neurol. 67:409.
  7. Jones, D.C. et al. (2012) Mol. Microbiol. 86:51.
  8. Li , M.H. et al. (2002) J. Biol. Chem. 277:46385.
  9. Wu, Z.L. (2011) PLoS ONE 6:e23172.

Alternate Names

C21orf124, C21orf97, HEL-S-1a, PDXK, PKH, PNK, Vitamin B6 Kinase

Entrez Gene IDs

8566 (Human); 216134 (Mouse); 83578 (Rat)

Gene Symbol

PDXK

UniProt

O00764

Additional Pyridoxal Kinase/PDXK Products

Product Documents for Recombinant Human Pyridoxal Kinase Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Pyridoxal Kinase Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.