Recombinant Human SALM4/LRFN3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5349-SA

R&D Systems, part of Bio-Techne
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5349-SA-050
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Key Product Details

Source

NS0

Accession #

Q9BTN0

Conjugate

Unconjugated

Applications

Bioactivity

View all SALM4/LRFN3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SALM4/LRFN3 protein
Ser17-Met539 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser17

Predicted Molecular Mass

56.3 kDa

SDS-PAGE

80-85 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
When 5 x 104 cells/well are added to rhSALM4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37 °C.  Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5349-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM4/LRFN3

Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region.  The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1 - 2).  In addition, there are five potential sites for N-linked glycosylation.  SALM4 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1-3.  Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4.  Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM4, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing Protein 3

Alternate Names

FIGLER1, LRFN3

Entrez Gene IDs

79414 (Human); 233067 (Mouse); 308495 (Rat)

Gene Symbol

LRFN3

UniProt

Q9BTN0

Additional SALM4/LRFN3 Products

Product Documents for Recombinant Human SALM4/LRFN3 Protein, CF

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Product Specific Notices for Recombinant Human SALM4/LRFN3 Protein, CF

For research use only

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