Recombinant Human SCF (Mammalian-expressed) Protein

R&D Systems, part of Bio-Techne | Catalog # 7466-SC

R&D Systems, part of Bio-Techne
Discontinued Product
7466-SC has been discontinued. An alternative/replacement product is available: 11010-SC. View all SCF/c-kit Ligand products.

Key Product Details

Source

HEK293

Accession #

P21583

Conjugate

Unconjugated

Applications

Bioactivity

View all SCF/c-kit Ligand Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein
Glu26-Ala189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu26

Predicted Molecular Mass

18.5 kDa (monomer)

SDS-PAGE

24-30 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 1-5 ng/mL.

Scientific Data Images for Recombinant Human SCF (Mammalian-expressed) Protein

Recombinant Human SCF (Mammalian-expressed) Protein Bioactivity

Recombinant Human SCF (Mammalian-expressed) Protein Bioactivity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL.

Formulation, Preparation and Storage

7466-SC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c‑kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O‑linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel‑dickie mutant mice (11, 12). An alternatively spliced isoform of human SCF exists that lacks 28 aa that encompass the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

References

  1. Ashman, L.K., 1999, Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al., 2000, Int. J. Dev. Biol. 44:599.
  3. Yoshida, H. et al., 2001, J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al., 2004, Exp. Cell Res. 301:201.
  5. Kapur, R. et al., 2002, Blood 100:1287.
  6. Wang, C.-H. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al., 2006, J. Cell Sci. 119:3039.
  8. Reber, L. et al., 2006, Eur. J. Pharmacol. 533:327.
  9. Martin, F.H. et al., 1990, Cell 63:203.
  10. Arakawa, T. et al., 1991, J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al., 1994, J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al., 1991, Proc. Natl. Acad. Sci. USA 88:4671.
  13. 13. Anderson, D.M. et al., 1991, Cell Growth Differ. 2:373.
  14. 14. Lemmon, M.A. et al., 1997, J. Biol. Chem. 272:6311.
  15. 15. Kanellakis, P. et al., 2006, Cardiovasc. Res. 70:117.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Gene Symbol

KITLG

UniProt

P21583

Additional SCF/c-kit Ligand Products

Product Documents for Recombinant Human SCF (Mammalian-expressed) Protein

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Product Specific Notices for Recombinant Human SCF (Mammalian-expressed) Protein

For research use only

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