Recombinant Human Semaphorin 4D Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7470-S4

R&D Systems, part of Bio-Techne
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7470-S4-050
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Key Product Details

Source

CHO

Accession #

Q92854

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 4D/CD100 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 4D/CD100 protein
Human Semaphorin 4D
(Met22-Arg734)
Accession # Q92854		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22 & Phe24

Predicted Molecular Mass

105.8 kDa (monomer)

SDS-PAGE

110-135 kDa, reducing conditions

Activity

Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells.
The ED50 for this effect is typically 0.4-2 μg/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 μg/mL, Recombinant Human Semaphorin 4D/CD100 Fc Chimera binds with an apparent Kd <4 nM.

Formulation, Preparation and Storage

7470-S4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D/CD100, previously Sem J, G or C-like 2) is a 150 kDa type I transmembrane glycoprotein that belongs to the Class 4 family of transmembrane immune and nervous system semaphorins (1‑3). The human Sema4D cDNA encodes 862 amino acids (aa) that include a 21 aa signal sequence, a 713 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 107 aa cytoplasmic tail. The Sema4D ECD contains one sema, PSI and immunoglobulin‑like domain, and over the full ECD (aa 22‑734), human Sema4D shares 85%, 85%, 86% and 80% aa sequence identity with mouse, rat, porcine and canine Sema4D ECDs, respectively. Sema4D is active as a homodimer that utilizes both a membrane‑proximal intermolecular disulfide bond and two hydrophobic interactions involving Phe244 and Phe246 (4, 5). Proteolysis by metalloproteinases such as TACE/ADAM17 and MMP-14 (MT1‑MMP) produce a soluble, active 120 kDa form that may also exist as a dimer (5‑7). Sema4D, produced by T cells, activated B cells and dendritic cells acts through a low affinity receptor termed CD72 in the immune system (1, 2). Unligated CD72 inhibits antigen presenting cells that express it, and this inhibition is relieved by Sema4D binding (8). Sema4D thus causes enhanced B cell survival, differentiation of macrophages and DC, and enhanced T cell antigen priming (2, 8, 9). In the CNS, Sema4D and a high affinity receptor Plexin B1 are expressed in complimentary patterns in several regions of the brain, and their interaction assists in the guidance of developing neurons (10). Intermediate affinity Sema4D:Plexin B2 binding also occurs during development, and enhances both radial and tangential neuronal migration (11). In cancer, Sema4D can also be produced by tumor‑associated macrophages, and bind to endothelial cell Plexin B1, thus promoting tumor angiogenesis (3, 6, 7, 12). While this suggests facilitation of the metastatic process, it is also recognized that semaphorins in general show both tumor promoter and suppressor functions (13, 14). For Sema4D, this is reflected in its ability to inhibit ovarian carcinoma cell survival. Finally, receptors that associate in cis with plexins, such as Met and ErbB2, are known to alter Sema4D‑mediated activities (3).

References

  1. Furuyama, T. et al. (1996) J. Biol. Chem. 271:33376.
  2. Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292.
  3. Ch’ng, E.S. and A. Kumanogoh (2010) Mol. Cancer 9:251.
  4. Janssen, B.J.C. et al. (2010) Nature 467:1118.
  5. Elhabazi, A. et al. (2001) J. Immunol. 166:4341.
  6. Zhu, L. et al. (2007) Proc. Natl. Acad. Sci. USA 104:1621.
  7. Basile, J.R. et al. (2007) J. Biol. Chem. 282:6899.
  8. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  9. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  10. Worzfield, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  11. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  12. Sierra, J.R. et al. (2008) J. Exp. Med. 205:1673.
  13. Ye, S. et al. (2010) BMC Cancer 10:611.
  14. Lu, T-P. et al. (2012) Transl. Cancer Res. 1:74.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Entrez Gene IDs

10507 (Human); 20354 (Mouse); 306790 (Rat)

Gene Symbol

SEMA4D

UniProt

Q92854

Additional Semaphorin 4D/CD100 Products

Product Documents for Recombinant Human Semaphorin 4D Fc Chimera Protein, CF

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