Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1146-S6

R&D Systems, part of Bio-Techne
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1146-S6-025
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Key Product Details

Source

NS0

Accession #

Q9H2E6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 6A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 6A protein
Human Semaphorin 6A
(Gly19-Thr649)
Accession # Q9H2E6		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly19

Predicted Molecular Mass

97 kDa (monomer)

SDS-PAGE

120 kDa, reducing conditions

Activity

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391.
The ED50 for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

1146-S6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta propeller N-terminal semaphorin (sema) domain and also an extracellular
plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, human Sema6A shares 94% and 93% aa sequence identity with mouse and rat Sema6A, respectively. Alternative splicing generates an additional isoform with a 17 aa insertion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4
(3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).

References

  1. Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292.
  2. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  3. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  4. Matsuoka, R.L. et al. (2011) Nature 470:259.
  5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
  6. Suto, F. et al. (2007) Neuron 53:535.
  7. Haklai-Topper, L. et al. (2010) EMBO J. 29:2635.
  8. Xu, X.M. et al. (2000) J. Neurosci. 20:2638.
  9. Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516.
  10. Mauti, O. et al. (2007) Neural Dev. 2:28.
  11. Segarra, M. et al. (2012) Blood 120:4104.
  12. Kigel, B. et al. (2011) Blood 118:4285.
  13. Bernard, F. et al. (2012) Glia 60:1590.
  14. Gautier, G. et al. (2006) Am. J. Pathol. 168:453.

Alternate Names

Sema6A

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Gene Symbol

SEMA6A

UniProt

Q9H2E6

Additional Semaphorin 6A Products

Product Documents for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

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