Recombinant Human Serpin A4/Kallistatin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1669-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Serpin A4/Kallistatin protein
Gln21-Pro427, with a C-terminal 10-His tag
Gln21-Pro427, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln21 predicted
Predicted Molecular Mass
48 kDa
SDS-PAGE
57 kDa and 61 kDa, reducing conditions
Activity
Measured by its ability to inhibit KLK1 cleavage of a fluorogenic peptide substrate, Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The IC50 value is <40 nM, as measured under the described conditions.
The IC50 value is <40 nM, as measured under the described conditions.
Reviewed Applications
Read 3 reviews rated 4.7 using 1669-PI in the following applications:
Formulation, Preparation and Storage
1669-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 50 mM Tris, 10 mM CaCl2 and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin A4/Kallistatin
Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
- Miao, R.Q. et al. (2002) Blood 100:3245.
Alternate Names
Kallistatin
Entrez Gene IDs
5267 (Human)
Gene Symbol
SERPINA4
UniProt
Additional Serpin A4/Kallistatin Products
Product Documents for Recombinant Human Serpin A4/Kallistatin Protein, CF
Product Specific Notices for Recombinant Human Serpin A4/Kallistatin Protein, CF
For research use only
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