Recombinant Human Serpin A5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1266-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Serpin A5/Protein C Inhibitor protein
His20-Pro406, with a C-terminal 10-His tag
His20-Pro406, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His20
Predicted Molecular Mass
45 kDa
SDS-PAGE
50-55 kDa, reducing conditions
Formulation, Preparation and Storage
1266-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin A5/Protein C Inhibitor
Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallikreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
- Geiger, M. et al. (1996) Immunopharmacology 32:53.
Alternate Names
PAI-3, PCI
Gene Symbol
SERPINA5
UniProt
Additional Serpin A5/Protein C Inhibitor Products
Product Documents for Recombinant Human Serpin A5 Protein, CF
Product Specific Notices for Recombinant Human Serpin A5 Protein, CF
For research use only
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