Recombinant Human Serpin A6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9580-PI

R&D Systems, part of Bio-Techne
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9580-PI-050
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Key Product Details

Source

NS0

Accession #

AAH56259.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Serpin A6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Serpin A6 protein
Met23-Val405, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met23

Predicted Molecular Mass

43 kDa

SDS-PAGE

50-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Serpin A6 binds with an ED50 of 1.5-7.5 μg/mL.

Scientific Data Images for Recombinant Human Serpin A6 Protein, CF

Recombinant Human Serpin A6 Protein Bioactivity

Recombinant Human Serpin A6 Protein Bioactivity

When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Serpin A6 (Catalog # 9580-PI) binds with an ED50 of 1.5-7.5 μg/mL.

Formulation, Preparation and Storage

9580-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Trehalose.
Reconstitution
Reconstitute at 1 mg/mL in water.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A6

Corticosteroid-binding globulin (CBG), also known as Serpin A6, is an approximately 42 kDa member of the serpin superfamily of serine protease inhibitors. CBG belongs to the clade A subgroup of serpins which include inflammatory response molecules (1). Human CBG shares 57% sequence identity to mouse CBG. CBG is one of two serpins known to bind and transport hormones. CBG is responsible for binding ~90% of cortisol in a reversible manner to regulate levels under normal physiological states (2). However, cleavage of CBG's reactive site loop by proteases present during an inflammatory response leads to a targeted and immediate release of the anti-inflammatory steroid, cortisol, from CBG (3). Reduced cleavage of CBG is implicated in the inflammatory phenotype seen in obesity, metabolic syndrome, and rheumatoid arthritis (4, 5). Cleavage of CBG can be modulated by glycosylation (6). Naturally occurring mutations of CBG lead to abnormal levels or function (7, 8). Loss of functional CBG results in low cortisol levels in patients experiencing chronic pain, fatigue, hypotension and excess weight (8).

References

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Lewis, J. G. et al (2005) Clin. Chem. Acta. 359:189.
  3. Klieber M.A. et al. (2007) J. Biol. Chem. 282:29594.
  4. Nenke, M.A. et al. (2016) Horm. Metab. Res. 48:523.
  5. Nenke, M.A. et al. (2016) Clin. Endocrinol. 85:369.
  6. Sumer-Bayraktar, Z. et al. (2016) J. Biol. Chem 291:17727.
  7. Simard, M. et al. (2015) J. Clin. Endocrinol. Metab. 100:E129.
  8. Gagliardi, L. et al. (2010) Mol. Cell. Endocrinol. 316:24.

Alternate Names

CBG

Entrez Gene IDs

866 (Human); 12401 (Mouse); 299270 (Rat)

Gene Symbol

SERPINA6

UniProt

AAH56259.1

Additional Serpin A6 Products

Product Documents for Recombinant Human Serpin A6 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Serpin A6 Protein, CF

For research use only

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