Recombinant Human SF20/MYDGF Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10231-MY

R&D Systems, part of Bio-Techne
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10231-MY-050
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Key Product Details

Source

CHO

Accession #

Q969H8

Conjugate

Unconjugated

Applications

Bioactivity

View all SF20/MYDGF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SF20/MYDGF protein
Val32-Leu173

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val32

Predicted Molecular Mass

16 kDa

SDS-PAGE

14-15 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SF20/MYDGF (Catalog # 10231-MY) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED50 of 0.8-4.8 μg/mL.

Scientific Data Images for Recombinant Human SF20/MYDGF Protein, CF

Recombinant Human SF20/MYDGF Protein Binding Activity

Recombinant Human SF20/MYDGF Protein Binding Activity

When Recombinant Human SF20/MYDGF (Catalog # 10231-MY) is immobilized at 2 µg/mL, 100 µg/well, Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED50 of 0.8-4.8 µg/mL.
Recombinant Human SF20/MYDGF Protein SDS-PAGE

Recombinant Human SF20/MYDGF Protein SDS-PAGE

2 μg/lane of Recombinant Human SF20/MYDGF (Catalog # 10231-MY) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-15 kDa.

Formulation, Preparation and Storage

10231-MY
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Human MYDGF shares 92% and 85% amino acid sequence identity to mouse and rat MYDGF, respectively. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

References

  1. Korf-Klingebiel, M. et al. (2015) Nat. Med. 10:3778.
  2. Wang, P. et al. (2004) Cell. Mol. Life Sci. 61:2405
  3. Sunagozaka, H. et al. (2011) Int. J. Cancer 129:1576
  4. Bortnov, V. et al. (2018) J. Biol. Chem. 293:13166.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human); 28106 (Mouse)

Gene Symbol

MYDGF

UniProt

Q969H8

Additional SF20/MYDGF Products

Product Documents for Recombinant Human SF20/MYDGF Protein, CF

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Product Specific Notices for Recombinant Human SF20/MYDGF Protein, CF

For research use only

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