Recombinant Human SHBG Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9614-SG

R&D Systems, part of Bio-Techne
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9614-SG-050
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Key Product Details

Source

NS0

Accession #

P04278-1

Conjugate

Unconjugated

View all SHBG Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SHBG protein
Leu30-His402, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu30

Predicted Molecular Mass

41 kDa

SDS-PAGE

41-52 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9614-SG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHBG

Human SHBG (Sex hormone-binding globulin), also known as ABP (Testis-specific Androgen-binding protein) or TeBG (Testosterone-estradiol-binding globulin), is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family (1). Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G- like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G- like domain (2). SHBG is synthesized by the liver and circulates in blood. It binds to certain androgens and estrogens and serves as a transporter for the bound steroids (3); it is also involved in receptor mediated processes (4). Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG. Low SHGB levels are associated with increased risk for type 2 diabetes for both man and women (5).

References

  1. Awakumov G.V. et al. (2010) Mol. Cell Endocrinol. 5:316.
  2. Grishkovskaya I. et al. (2000) EMBO J. 19:504.
  3. Anderson D.C. (1974) Clin. Endocrinol. (Oxf.) 3:69.
  4. Rosner W. et al. (2010) Mol. Cell Endocrinol.5:795.
  5. Muka T. et al. (2017) Diabetes. 66:577.

Long Name

Sex Hormone-binding Globulin

Alternate Names

ABP

Entrez Gene IDs

6462 (Human)

Gene Symbol

SHBG

UniProt

P04278-1

Additional SHBG Products

Product Documents for Recombinant Human SHBG Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human SHBG Protein, CF

For research use only

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