Recombinant Human Sirtuin 1/SIRT1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7714-DA
Key Product Details
Product Specifications
Source
E. coli-derived human Sirtuin 1/SIRT1 protein
Met1-Ser747, with a C-terminal 6-His tag.
Met1-Ser747, with a C-terminal 6-His tag.
Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
82 kDa
SDS-PAGE
110-113 kDa, reducing conditions
Activity
Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016) in a coupled assay.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
7714-DA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Sirtuin 1/SIRT1
References
- Langley, E. et al. (2002) EMBO J. 21:2383.
- Chen, W. Y. et al. (2005) Cell 123:437.
- Huffmun, D. M. et al. (2007) Cancer Res. 67:6612.
- Mattagajasingh, I. et al. (2007) Proc. Natl. Acad. Sci. 104:14855.
- Chen, J. et al. (2005) J. Biol. Chem. 280:40364.
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1
Gene Symbol
SIRT1
UniProt
Additional Sirtuin 1/SIRT1 Products
Product Documents for Recombinant Human Sirtuin 1/SIRT1 Protein, CF
Product Specific Notices for Recombinant Human Sirtuin 1/SIRT1 Protein, CF
For research use only
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