Recombinant Human Sirtuin 3/SIRT3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7488-DA
Key Product Details
Product Specifications
Source
E. coli-derived human Sirtuin 3/SIRT3 protein
Ser101-Lys399, with an N-terminal Met and 6-His tag
Ser101-Lys399, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
34 kDa
SDS-PAGE
30-33 kDa, reducing conditions
Activity
Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016) in a coupled assay.
The specific activity is >2 pmol/min/μg, as measured under the described conditions.
The specific activity is >2 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
7488-DA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Sirtuin 3/SIRT3
References
- North, B. J. et al. (2004) Genome Biology 5:224.
- Schwer, B. et al. (2006) Proc. Natl. Acad. Sci. 103:11224.
- Schlicker, C. et al. (2008) J. Mol. Biol. 382:790.
- Ahn, B.-H. et al. (2008) Proc. Natl. Acad. Sci. 105:14447.
- Jin, L. et al. (2009) J. Biol. Chem. 284: 24394.
- Rose, G. et al. (2003) Exp. Gerontol. 38: 1065.
Long Name
Silent Mating Type Information Regulation 3 Homolog
Alternate Names
SIR2-like protein 3, SIR2L3, SIRT3
Gene Symbol
SIRT3
UniProt
Additional Sirtuin 3/SIRT3 Products
Product Documents for Recombinant Human Sirtuin 3/SIRT3 Protein, CF
Product Specific Notices for Recombinant Human Sirtuin 3/SIRT3 Protein, CF
For research use only
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