Recombinant Human Slit2 (aa1122-1529) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9379-SL

R&D Systems, part of Bio-Techne
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9379-SL-050
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Key Product Details

Source

HEK293

Accession #

O94813-1

Conjugate

Unconjugated

Applications

Bioactivity

View all Slit2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Slit2 protein
Thr1122-Ser1529, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr1122

Predicted Molecular Mass

45 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Slit2, immobilized at 625 ng/ml on a 96-well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human Slit2 (aa1122-1529) Protein, CF

Recombinant Human Slit2 (aa1122-1529) Protein Bioactivity

Recombinant Human Slit2 (aa1122-1529) Protein Bioactivity

Recombinant Human Slit2 (Catalog # 9379-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 625 ng/mL of Recombinant Human Slit2.

Formulation, Preparation and Storage

9379-SL
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Slit2

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is widely expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including seven EGF-like domains, twenty Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and four N-terminal and four C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature human Slit2 shares 96% amino acid sequence identity with the mouse and rat orthologs. Slit2 has been shown to have various important functions in the nervous system. Slit2 induces growth cone collapse, inhibits oligodendrocyte precursor cell migration, and promotes axon elongation, branch formation, and fasciculation (5-9). Slit2 C-terminal fragment can mediate axon guidance through binding to Plexin A1 receptor (10). Slit2-C has also been shown to bind to Glypican-1 and promote motor axon migration (5). Outside the nervous system, C-terminal fragment of Slit2 activates a thermogenic PKA pathway in adipocytes and improves glucose homeostasis (11).

References

  1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
  2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
  3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
  4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
  5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  6. Wang, K.H. et al. (1999) Cell 96:771.
  7. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
  8. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
  9. Jaworski, A. and Tessier-Lavigne, M. (2012) Nat. Neurosci. 15:367.
  10. Delloye-Bourgeois, C. et.al. (2014) Nat. Neurosci. 18(1):36.
  11. Svensson, K.J. et al. (2016) Cell Metabolism. 23:1.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

O94813-1

Additional Slit2 Products

Product Documents for Recombinant Human Slit2 (aa1122-1529) Protein, CF

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Product Specific Notices for Recombinant Human Slit2 (aa1122-1529) Protein, CF

For research use only

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