Recombinant Human SLITRK6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8985-SK

R&D Systems, part of Bio-Techne
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8985-SK-050
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Key Product Details

Source

NS0

Accession #

Q9H5Y7

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLITRK6 protein
Ile16-Ala604, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile16 & His19

Predicted Molecular Mass

66 kDa

SDS-PAGE

72-95 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

8985-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK6

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region. Mature human SLITRK6 consists of a 582 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, human SLITRK6 shares 88% aa sequence identity with mouse and rat SLITRK6. SLITRK6 is expressed in multiple regions of the developing nervous system, notably in the otic vesicle, cochlear and vestibular sensory epithelia, and spiral and vestibular ganglion neurons (3, 4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (4, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

References

  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  3. Aruga, J. (2003) Gene Expr. Patterns 3:727.
  4. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
  7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.

Long Name

SLIT and NTRK-like Family, Member 6

Alternate Names

FLJ22774, MGC119595, MGC119596, MGC119597,4832410J21Rik, SLIT and NTRK-like family, member 6, SLIT and NTRK-like protein 6, slit and trk like gene 6

Entrez Gene IDs

84189 (Human); 239250 (Mouse); 290467 (Rat)

Gene Symbol

SLITRK6

UniProt

Q9H5Y7 (Human)

Additional SLITRK6 Products

Product Documents for Recombinant Human SLITRK6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SLITRK6 Protein, CF

For research use only

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