Recombinant Human SLPI Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1274-PI
Key Product Details
Product Specifications
Source
E. coli-derived human SLPI protein
Ser26-Ala132
Ser26-Ala132
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser26
Predicted Molecular Mass
12 kDa
SDS-PAGE
13 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <4.0 nM, under the described conditions.
The IC50 value is <4.0 nM, under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1274-PI in the following applications:
Formulation, Preparation and Storage
1274-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: SLPI
Secretory Leukocyte Protease Inhibitor (SLPI) is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid. The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin. SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids. It could be used as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.
Long Name
Secretory Leukocyte Protease Inhibitor
Alternate Names
ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI
Gene Symbol
SLPI
UniProt
Additional SLPI Products
Product Documents for Recombinant Human SLPI Protein, CF
Product Specific Notices for Recombinant Human SLPI Protein, CF
For research use only
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