Recombinant Human SOST/Sclerostin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10962-ST

R&D Systems, part of Bio-Techne
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10962-ST-050
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Key Product Details

Source

CHO

Accession #

Q9BQB4.1

Conjugate

Unconjugated

Applications

Bioactivity

View all SOST/Sclerostin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SOST/Sclerostin protein
Gln24-Tyr213, with a N-terminal 7-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His of Tag

Predicted Molecular Mass

22 kDa

SDS-PAGE

25-35 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 1.50-6.00 μg/mL.

Scientific Data Images for Recombinant Human SOST/Sclerostin His-tag Protein, CF

Recombinant Human SOST/Sclerostin His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human SOST/Sclerostin His-tag Protein (Catalog # 10962-ST) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualizedby Coomassie® Blue staining, showing bands at 25-35 kDa.

Formulation, Preparation and Storage

10962-ST
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

Sclerostin (SOST) is a member of the Cerberus/differential screening-selected gene in neuroblastoma (DAN) family, a group of secreted glycoproteins characterized by a cysteine-knot motif. The Cerberus/DAN family consists of multiple members originally identified as putative BMP antagonists including Dan, SOST, Cerberus, Gremlin, USAG-1, PRDC, and Coco (1, 2). While the overall sequence identity between family members is low, they have conserved spacing of six cysteine residues in the C-terminus which form the structurally conserved cysteine-knot motif. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer (1). Mature human SOST shares 90% amino acid sequence identity with mouse and rat SOST. SOST appears to be the first example of a BMP antagonist predominately localized to osteoclasts and functions as an important regulator of bone homeostasis by inhibiting bone mineralization (3). SOST has been shown to have unique ligand specificity by binding directly to BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity thereby repressing BMP-induced osteogenesis (4). Mutations in the SOST gene can cause several bone dysplasia disorders characterized by progressive skeletal overgrowth including sclerosteosis and van Buchem disease (5). Additionally, SOST has been shown to be a strong Wnt antagonist by directly binding the Wnt co-receptors LRP5 and LRP6 (6).

References

  1. Nolan, K. and Thompson, T.B. (2014) Protein Sci. 23:999.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. van Bezooijen, R.L. et al. (2004) J. Exp. Med. 199:805.
  4. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  5. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
  6. Kim, J. et al. (2020) Nat Commun 11:5357.

Alternate Names

sclerostin, VBCHsclerosteosis

Entrez Gene IDs

50964 (Human); 74499 (Mouse)

Gene Symbol

SOST

UniProt

Q9BQB4.1

Additional SOST/Sclerostin Products

Product Documents for Recombinant Human SOST/Sclerostin His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SOST/Sclerostin His-tag Protein, CF

For research use only

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