Recombinant Human SPINK1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7496-PI
Key Product Details
Product Specifications
Source
E. coli-derived human SPINK1 protein
Asp24-Cys79, with an N-terminal Met and 6-His tag
Asp24-Cys79, with an N-terminal Met and 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
7 kDa
SDS-PAGE
8-11 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <10 nM, as measured under the described conditions.
The IC50 value is <10 nM, as measured under the described conditions.
Formulation, Preparation and Storage
7496-PI
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2, DTT, Glycerol and Brij-35. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: SPINK1
References
- Deybach J.C.D. et al. (2003) Hum. Genet. 113:369.
- Chandak G.R. et al. (2002) J. Med. Genet. 39:347.
- Huhtala M.L. et al. (1982) J. Biol. Chem. 257:13713.
Long Name
Serine Peptidase Inhibitor, Kazal Type 1
Alternate Names
PCTT, PSTI, Spink3, TATI
Gene Symbol
SPINK1
UniProt
Additional SPINK1 Products
Product Documents for Recombinant Human SPINK1 Protein, CF
Product Specific Notices for Recombinant Human SPINK1 Protein, CF
For research use only
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