Recombinant Human ST6GALNAC1 Protein

R&D Systems, part of Bio-Techne | Catalog # 9154-GT

R&D Systems, part of Bio-Techne
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9154-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

HEK293

Accession #

Q9NSC7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST6 Sialyltransferase 1/ST6GALNAC1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ST6 Sialyltransferase 1/ST6GALNAC1 protein
Lys36-Asn600, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys36 & Thr73

Predicted Molecular Mass

65 kDa

SDS-PAGE

70-89 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >90 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9154-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST6 Sialyltransferase 1/ST6GALNAC1

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid‑containing molecules. Alpha‑N‑acetylgalactosaminide alpha ‑2,6‑sialyltransferase 1, or ST6GALNAC1, is a type II membrane protein localized in the Golgi network and catalyzes 2,6‑sialylation of the Tn antigen (GalNAc‑O‑Ser/Thr) (1, 2). Sialyl‑Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis (3). Breast cancer cells transfected with ST6GALNAC1 synthesized the sialyl‑Tn antigen structure (4). The enzyme may also be used to synthesize a cancer vaccine (5). The activity of this enzyme has been measured using a phosphatase‑coupled assay (6).

References

  1. Ikehara, Y, et al. (1999) Glycobiology 9:1213.
  2. Sewell, R. et al. (2006) J. Biol. Chem. 281:3586.
  3. Lima, L. et al. (2013) Br. J. Cancer 109:2106.
  4. Julien, S. et al. (2001) Glycoconj. J. 18:883.
  5. Julien, S. et al. (2009) Br. J. Cancer. 100:1746.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 1

Alternate Names

Siat7A, ST6GalNAcI, STYI

Entrez Gene IDs

55808 (Human); 20445 (Mouse); 287920 (Rat)

Gene Symbol

ST6GALNAC1

UniProt

Q9NSC7

Additional ST6 Sialyltransferase 1/ST6GALNAC1 Products

Product Documents for Recombinant Human ST6GALNAC1 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST6GALNAC1 Protein

For research use only

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