Recombinant Human ST8SIA2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6590-GT

R&D Systems, part of Bio-Techne
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6590-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

Q92186

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 protein
Asp24-Thr375, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No sequence observed. Protein identity confirmed by detection of His-tag using Western analysis.

Predicted Molecular Mass

41 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its ability to transfer sialic acid from CMP-NeuAc to Recombinant Human NCAM-1/CD56 120 isoform (Catalog # 2408-NC).
The specific activity is >85 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6590-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2

Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).

References

  1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
  2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
  3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
  4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
  5. Ong, E. et al. (1998). Glycobiology 8:415.
  6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
  7. Thompson, M. G. et al. (2011) J. Biol. Chem. 286:4525.
  8. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2

Alternate Names

SIAT8B, ST8SiaII, STX

Entrez Gene IDs

8128 (Human)

Gene Symbol

ST8SIA2

UniProt

Q92186

Additional ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Products

Product Documents for Recombinant Human ST8SIA2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST8SIA2 Protein, CF

For research use only

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