Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9405-SO

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9405-SO-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

O76061

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Stanniocalcin 2/STC-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Stanniocalcin 2/STC-2 protein
Thr25-Arg302, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

31 kDa

SDS-PAGE

34-43 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

Recombinant Human Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Human Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

9405-SO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 2/STC-2

Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),

References

  1. Luo, C. et al. (2005). Endocrinology 146:469.
  2. Tagliabracci, V. S. et al. (2015). Cell 161:1619.
  3. Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
  4. Kim PH, et al. (2015) BMB Rep. 48:702.
  5. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
  6. Hou J, et al. (2015) PLoS One. 10:e0122179.
  7. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
  8. Wu S, et al. (2006) J Biol Chem. 281: 5120.
  9. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
  10. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

Alternate Names

STC-2, STC2, STCRP

Entrez Gene IDs

8614 (Human); 20856 (Mouse)

Gene Symbol

STC2

UniProt

O76061

Additional Stanniocalcin 2/STC-2 Products

Product Documents for Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.