Recombinant Human Sulfamidase/SGSH Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8380-SU

R&D Systems, part of Bio-Techne
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8380-SU-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P51688

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Sulfamidase/SGSH Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sulfamidase/SGSH protein
Arg23-Leu502 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg23

Predicted Molecular Mass

55 kDa

SDS-PAGE

55-63 kDa, under reducing conditions.

Activity

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >0.125 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human Sulfamidase/SGSH Protein, CF

Recombinant Human Sulfamidase/SGSH Protein Enzyme Activity Diagram.

Recombinant Human Sulfamidase/SGSH Protein, CF (Catalog # 8380-SU) hydrolyzes N-linked sulfate group from the non-reducing terminal glucosaminide residues of heparan sulfate.

Recombinant Human Sulfamidase/SGSH Protein SDS-PAGE.

2 μg/lane of Recombinant Human Sulfamidase/SGSH Protein (Catalog # 8380-SU) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-63 kDa.

Formulation, Preparation and Storage

8380-SU
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Sulfamidase/SGSH

Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (HS) (1). Different from the HS specific endosulfatases that remove sulfate from internal GlcNAc residues (2), SGSH removes sulfate group from the non-reducing end glucosamine residues on HS. The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (3). Human SGSH shows 88.6% sequence identity with that of mouse sequence.

References

  1. DiezRoux, G. and Ballabio, A. et al. (2005) Annu. Rev. Genomics Hum. Genet. 6:355.
  2. Morimoto-Tomita, M. et al. (2002) J. Biol. Chem. 277:49175.
  3. Blanch, L. et al. (1997) Hum. Mol. Genet. 6:787.

Alternate Names

HSS, MPS3A, SFMD, SGSH

Entrez Gene IDs

6448 (Human); 27029 (Mouse)

Gene Symbol

SGSH

UniProt

P51688

Additional Sulfamidase/SGSH Products

Product Documents for Recombinant Human Sulfamidase/SGSH Protein, CF

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Product Specific Notices for Recombinant Human Sulfamidase/SGSH Protein, CF

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