Recombinant Human SULT1E1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5545-ST

R&D Systems, part of Bio-Techne
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5545-ST-020
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Key Product Details

Source

E. coli

Accession #

P49888

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 1E1/SULT1E1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 1E1/SULT1E1 protein
Asn2-Ile294, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

36 kDa

SDS-PAGE

35 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to 1-Napthol.
The specific activity is >40 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5545-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5). The enzymatic activity of the recombinant human SULT1E1 is measured using a phosphatasecoupled assay (6).

References

  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

EST-1, STE, SULT1E1

Entrez Gene IDs

6783 (Human); 20860 (Mouse); 25355 (Rat)

Gene Symbol

SULT1E1

UniProt

P49888

Additional Cytosolic Sulfotransferase 1E1/SULT1E1 Products

Product Documents for Recombinant Human SULT1E1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human SULT1E1 Protein, CF

For research use only

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