Recombinant Human t-Plasminogen Activator/tPA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7449-SE

R&D Systems, part of Bio-Techne
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7449-SE-010
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Key Product Details

Source

CHO

Accession #

P00750

Conjugate

Unconjugated

Applications

Enzyme Activity

View all t-Plasminogen Activator/tPA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human t-Plasminogen Activator/tPA protein
Met1-Pro562, with a C-terminal 6-His tag
Single-chain proform was expressed, purified, activated with thermolysin to a 2-chain protease and further purified.

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser36 (Chain A) and Ile311 (Chain B)

Predicted Molecular Mass

31 kDa (Chain A), 29 kDa (Chain B)

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
The specific activity is >190 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 7449-SE in the following applications:

Scientific Data Images for Recombinant Human t-Plasminogen Activator/tPA Protein, CF

Recombinant Human t-Plasminogen Activator/tPA Protein Enzyme Activity

Recombinant Human t-Plasminogen Activator/tPA Protein Enzyme Activity

Recombinant Human t-Plasminogen Activator (Catalog # 7449-SE) is measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).

Formulation, Preparation and Storage

7449-SE
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: t-Plasminogen Activator/tPA

PLAT, also known as tissue-type plasminogen activator (tPA), is a secreted serine protease synthesized by endothelial cells (1).  The partially active single chain can be further processed to full activity by plasmin, tissue kallikrein or Factor Xa.  Active PLAT converts plasminogen to plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in plasminogen.  Unusually high levels of tPA activity can result in excessive bleeding, and low levels of tPA activity can result in thrombosis or embolism.  Human PLAT contains 4 domains; the N-terminal fibronectin type-1 domain, an epidermal growth factor-like domain, two kringle domains and a serine protease catalytic domain.

References

  1. Lijnen H.R. and D. Collen (2004) Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1684, Academic Press, San Diego.

Long Name

Tissue Plasminogen Activator

Alternate Names

Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator

Entrez Gene IDs

5327 (Human); 18791 (Mouse); 25692 (Rat)

Gene Symbol

PLAT

UniProt

P00750

Additional t-Plasminogen Activator/tPA Products

Product Documents for Recombinant Human t-Plasminogen Activator/tPA Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human t-Plasminogen Activator/tPA Protein, CF

For research use only

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