Recombinant Human TDO2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9768-TD
Tryptophan 2,3-dioxygenase
Key Product Details
Product Specifications
Source
E. coli-derived human TDO2 protein
Leu18-Phe388
with an N-terminal Met and a C-terminal 6-His tag
Leu18-Phe388
with an N-terminal Met and a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
45 kDa
SDS-PAGE
40 kDa and 78 kDa, reducing conditions
Activity
Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine.
The specific activity is >650 pmol/min/μg, as measured under the described conditions.
The specific activity is >650 pmol/min/μg, as measured under the described conditions.
Scientific Data Images for Recombinant Human TDO2 Protein, CF
Recombinant Human TDO2 Protein Enzyme Activity
Recombinant Human TDO2 (Catalog # 9768-TD) is measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The activity (orange) is approximately 10-fold greater than the competitor's TDO2 (green).
Recombinant Human TDO2 Protein SDS-PAGE
1 μg/lane of Recombinant Human TDO2 (Catalog # 9768-TD) and 1 μg/lane of competitor Human TDO2 was resolved with 4‑20% SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining.Formulation, Preparation and Storage
9768-TD
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TDO2
Tryptophan 2,3-dioxygenase (TDO2), a heme-containing cytosolic dioxygenase, forms a homo-tetrameric active molecule of approximately 190 kDa composed of 48 kDa monomers (1, 2). Human TDO2 shares 89% aa sequence identity with mouse TDO2. TDO2 is one of three proteins capable of catalyzing the first and rate-limiting step of the L-kynurenine pathway (KP): oxidative cleavage of the essential amino acid L-tryptophan to form N‑formyl‑kynurenine (3). TDO2 is a cytosolic protein typically localized to the liver and brain, unlike the more ubiquitously expressed indoleamine 2,3-dioxygenase (IDO), yet it is responsible for ~90% of the primary route of catabolism of tryptophan through the KP (3). TDO2 is upregulated in extrahepatic tumors (4-6) and is consequently a target in cancer immunotherapy (7). TDO2 is a therapeutic target in brain disease such as schizophrenia, Alzheimers disease, multiple sclerosis and glioma (8-11) due to its role in the regulation of levels of critical biologically active downstream KP metabolites (3). Polymorphisms in the TDO2 gene have been implicated for a role in behavioural responses and autism (12,13).
References
- Lewis-Ballester, A. et al. (2016) Sci. Rep. 6:35169.
- Rafice, S.A. et al. (2009) Biochem. Soc. Trans. 37:408.
- Badawy, A. (2017) Int J. Tryptophan Res. 10:1.
- Pilotte, L. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:2497.
- D'Amato, N.C. et al. (2015) Cancer Res. 75:4651.
- Yu, C.P et al. (2017) Med. Oncol. 34:73.
- Platten, et al. (2015) Front. Immunol. 5:673.
- Breda, C. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113:5435.
- Yu, C.P. et al. (2016) Metab. Brain Dis. 31:737.
- Lanz. T.V. et al. (2017) Sci. Rep. 7:41271.
- Reus, G.Z. et al. (2018) Prog. Neuropsychopharmacol. Biol. Psychiatry 81:55.
- Soichot, M. et al. (2013) Alcohol Alcohol 48:415.
- Nabi, R. et al. (2004) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 125B:63.
Long Name
Tryptophan 2,3-dioxygenase
Alternate Names
chky, TDO, TO, TRPO, Tryptophan Oxygenase, Tryptophan Pyrrolase, Tryptophanase
Gene Symbol
TDO2
UniProt
Additional TDO2 Products
Product Documents for Recombinant Human TDO2 Protein, CF
Product Specific Notices for Recombinant Human TDO2 Protein, CF
For research use only
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