Recombinant Human TFF2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8290-TF

R&D Systems, part of Bio-Techne
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8290-TF-050
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Key Product Details

Source

CHO

Accession #

Q03403

Conjugate

Unconjugated

Applications

Bioactivity

View all TFF2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TFF2 protein
Glu24-Tyr129

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

12 kDa

SDS-PAGE

17-25 kDa, reducing conditions

Activity

Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells.
5-15 μg/mL of Recombinant Human TFF2 can effectively induce ERK1/2 phosphorylation.

Formulation, Preparation and Storage

8290-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF2

Trefoil Factor 2 (TFF2), also known as spasmolytic peptide (SP), is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF2 is an approximately 20 kDa glycosylated peptide that plays an important role in epithelial regeneration and wound healing (1, 2). Mature human TFF2 shares 87% and 83% amino acid sequence identity with mouse and rat TFF2, respectively. TFF2 is primarily expressed by gastric mucosa of the pyloric stomach where it binds to Gastrokine 2 (3, 4). It is up-regulated in bronchiolar epithelium following exposure to allergens and is excreted into the urine at increased levels in patients with kidney stones (5, 6). TFF2 knockout mice exhibit increased gastric epithelium damage following H. pylori infection or treatment with non-steroidal anti-inflammatory drugs (7, 8). Administration of TFF2 can reduce the severity of experimental colitis (9-11). TFF2 is down-regulated in many gastric cancers, although it is up-regulated in some breast cancers (12-14). TFF2 promotes the migration of normal epithelial cells as well as tumor cells (14, 15).

References

  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. May, F.E.B. et al. (2000) Gut 46:454.
  3. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505.
  4. Otto, W.R. et al. (2006) Proteomics 6:4235.
  5. Nikolaidis, N.M. et al. (2006) Exp. Lung Res. 32:483.
  6. Rinnert, M. et al. (2010) Cell Tissue Res. 339:639.
  7. Fox, J.G. et al. (2007) Am. J. Pathol. 171:1520.
  8. Farrell, J.J. et al. (2002) J. Clin. Invest. 109:193.
  9. Soriano-Izquierdo, A. et al. (2004) J. Leukoc. Biol. 75:214.
  10. Poulsen, S.S. et al. (2005) Regul. Pept. 126:163.
  11. Tran, C.P. et al. (1999) Gut 44:636.
  12. Leung, W.K. et al. (2002) J. Pathol. 197:582.
  13. Kirikoshi, H. and M. Katoh (2002) Int. J. Oncol. 21:655.
  14. May, F.E. et al. (2004) Peptides 25:865.
  15. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.

Long Name

Trefoil Factor 2

Alternate Names

SML1, SP, Spasmolysin, Spasmolytic Polypeptide

Entrez Gene IDs

7032 (Human); 21785 (Mouse); 116592 (Rat)

Gene Symbol

TFF2

UniProt

Q03403

Additional TFF2 Products

Product Documents for Recombinant Human TFF2 Protein, CF

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Product Specific Notices for Recombinant Human TFF2 Protein, CF

For research use only

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