Recombinant Human TFPI-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2545-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TFPI-2 protein
Asp23-Lys213, with a C-terminal 10-His tag
Asp23-Lys213, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp23
Predicted Molecular Mass
23 kDa
SDS-PAGE
28-35 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <5 nM, as measured under the described conditions.
The IC50 value is <5 nM, as measured under the described conditions.
Formulation, Preparation and Storage
2545-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Brij-35. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TFPI-2
Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C‑terminal basic region (1-3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified rhTFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # http://www.rndsystems.com/product_results.aspx?k=2338-SE">2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # http://www.rndsystems.com/product_results.aspx?k=2339-PA">2339-PA) .
References
- Miyagi, Y. et al. (1994) J. Biochem. 116:939.
- Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
- Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
- Rollin, J. et al. (2005) Br, J. Cancer 92:775.
Long Name
Tissue Factor Pathway Inhibitor 2
Alternate Names
PP5, REF-1, TFPI2
Entrez Gene IDs
7980 (Human)
Gene Symbol
TFPI2
UniProt
Additional TFPI-2 Products
Product Documents for Recombinant Human TFPI-2 Protein, CF
Product Specific Notices for Recombinant Human TFPI-2 Protein, CF
For research use only
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