Recombinant Human TGF-beta RII Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 341-BR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TGF-beta RII protein
| Human TGF-beta RII (Thr23-Asp159) Accession # P37173 |
MD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr23
Predicted Molecular Mass
41.7 kDa (monomer)
SDS-PAGE
59 kDa-66 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human TGF‑ beta1 (Catalog # 240-B/CF) is immobilized at 0.5 µg/mL, the concentration of Recombinant Human TGF‑ beta RII Fc Chimera that produces 50% of the optimal binding response is found to be approximately 6-30 ng/mL.
When Recombinant Human TGF‑ beta1 (Catalog # 240-B/CF) is immobilized at 0.5 µg/mL, the concentration of Recombinant Human TGF‑ beta RII Fc Chimera that produces 50% of the optimal binding response is found to be approximately 6-30 ng/mL.
Formulation, Preparation and Storage
341-BR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute with PBS to prepare a stock solution of 100 μg/mL. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.
References
- Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Alternate Names
TGF-bRII, TGFbetaRII, TGFBR2
Gene Symbol
TGFBR2
UniProt
Additional TGF-beta RII Products
Product Documents for Recombinant Human TGF-beta RII Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human TGF-beta RII Fc Chimera Protein, CF
For research use only
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