Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 1003-RT

R&D Systems, part of Bio-Techne
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1003-RT-050
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Key Product Details

Source

NS0

Accession #

NP_001020018

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TGF-beta RII Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TGF-beta RII protein
Human TGF‑ beta RII Isoform 2
(Thr23-Asp184) &
(Ile24-Asp184)
Accession # NP_001020018		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23 & Ile24

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Measured by its ability to inhibit TGF-beta 1 activity on HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 1-3 ng/mL in the presence of 0.1 ng/mL of recombinant human TGF-beta 1.

Formulation, Preparation and Storage

1003-RT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70‑85 kDa) and Type III (250‑350 kDa). The Type III receptor, a proteoglycan that exists in membrane‑bound and soluble forms, binds TGF-beta 1, TGF-beta 2 and TGF-beta 3, and appears to participate in both TGF-beta dependent and independent cellular signaling. The Type II receptor, by contrast, is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity, and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors. TGF-beta receptor II isoform 2 (also TGF-beta RII isoform A) is an alternatively spliced variant of the standard Type II TGF-beta receptor (or TGF-beta RII isoform 1) that possesses a 27 amino acid substitution for Val10 near the N-terminus of the mature protein. Both T betaRII and T betaRII isoform 2 bind TGF-beta 1 and TGF-beta 3 with high affinity. However, only TGF-beta RII isoform 2 also binds TGF-beta 2 with high affinity in the absence of TGF‑ beta RIII. While TGF-beta RII is widely expressed on cells, TGF-beta RII isoform 2 shows distinct expression pattern mainly associated with bone‑related cells, such as osteoblasts and mesenchymal precursor cells. TGF-beta RII isoform 2 may play an important role in TGF-beta 2 binding and signaling in cells lacking TGF-beta RIII.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

NP_001020018

Additional TGF-beta RII Products

Product Documents for Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF

For research use only

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