Recombinant Human THSD7A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9524-TH

R&D Systems, part of Bio-Techne
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9524-TH-050
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Key Product Details

Source

HEK293

Accession #

Q9UPZ6

Conjugate

Unconjugated

Applications

Bioactivity

View all THSD7A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human THSD7A protein
Ala48-Trp1607, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala48

Predicted Molecular Mass

176 kDa

SDS-PAGE

160-265 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 0.1-0.6 μg/mL.

Scientific Data Images for Recombinant Human THSD7A Protein, CF

Recombinant Human THSD7A Protein Bioactivity

Recombinant Human THSD7A Protein Bioactivity

Immobilized Recombinant Human THSD7A (Catalog # 9524-TH) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.6 μg/mL.

Formulation, Preparation and Storage

9524-TH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THSD7A

Thrombospondin type I domain containing 7A, also known as THSD7A, is an approximately 250 kDa type I membrane protein (1). THSD-7A has a large extracellular domain containing ten thrombospondin type 1 repeats, six WSXW motifs, one RGD motif, and fourteen predicted N‑glycosylated sites (1). Mature THSD7A shares 91% and 92% amino acid sequence identity with mouse and rat, respectively. THSD7A is expressed in podocytes, glomerular endothelial cells and mesangial cells (2). It is a novel neural protein known to affect endothelial migration and vascular patterning during development (1, 3). Soluble THSD7A promotes endothelial filopodia formation and focal adhesion assembly and induces FAK-dependent signaling during angiogenesis (1). THSD7A can co-localize with aVb3 integrin in HUVECs (3). Additionally, most recent study has indicated that THSD7A is associated with obesity (4).

References

  1. Kuo, M.W. et al. (2011) PLoS One 6: e29000.
  2. Tomas, N.M. et al. (2014) N. Engl. J. Med. 371:2277.
  3. Wang, C.H. et al. (2010) J. Cell Physiol. 222: 685.
  4. Nizamuddin, S. et al. (2015) Int. J. Obes. (Lond.) 39:1662.

Long Name

Thrombospondin, Type I, Domain Containing 1

Alternate Names

Gm837, KIAA0960

Entrez Gene IDs

221981 (Human); 330267 (Mouse); 500032 (Rat)

Gene Symbol

THSD7A

UniProt

Q9UPZ6

Additional THSD7A Products

Product Documents for Recombinant Human THSD7A Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human THSD7A Protein, CF

For research use only

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