Recombinant Human Tissue alpha-L-Fucosidase/FUCA1, CF

R&D Systems, part of Bio-Techne | Catalog # 7039-GH

R&D Systems, part of Bio-Techne
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7039-GH-010
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

P04066

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Tissue alpha-L-Fucosidase/FUCA1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Tissue alpha-L-Fucosidase/FUCA1 protein
Gln32-Lys466 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln32

Predicted Molecular Mass

51 kDa

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-alpha -L-fucopyranoside.
The specific activity is >4,800 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7039-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

References

  1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
  2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
  4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
  5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
  6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
  7. O’Brien, J.S. et al. (1987) Enzyme 38:45.
  8. Durand, P. et al. (1969) J. Pediatr. 75:665.
  9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
  10. Shah, M. et al. (2008) Cancer 113:336.

Alternate Names

FUCA1, Tissue alphaLFucosidase

Entrez Gene IDs

2517 (Human); 71665 (Mouse); 24375 (Rat)

Gene Symbol

FUCA1

UniProt

P04066

Additional Tissue alpha-L-Fucosidase/FUCA1 Products

Product Documents for Recombinant Human Tissue alpha-L-Fucosidase/FUCA1, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Tissue alpha-L-Fucosidase/FUCA1, CF

For research use only

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