Recombinant Human TMIGD2/CD28H Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8316-TR

R&D Systems, part of Bio-Techne
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8316-TR-050
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Key Product Details

Source

HEK293

Accession #

Q96BF3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TMIGD2/CD28H Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TMIGD2/CD28H protein
Human TMIGD2
(Leu23-Gly150)
Accession # Q96BF3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

57-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TMIGD2/CD28H Fc Chimera is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human B7-H7  Fc Chimera (Catalog # 8084-B7) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL.

Formulation, Preparation and Storage

8316-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Entrez Gene IDs

126259 (Human)

Gene Symbol

TMIGD2

UniProt

Q96BF3

Additional TMIGD2/CD28H Products

Product Documents for Recombinant Human TMIGD2/CD28H Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TMIGD2/CD28H Fc Chimera Protein, CF

For research use only

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