Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera Protein
R&D Systems, part of Bio-Techne | Catalog # 630-TR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TRAIL R3/TNFRSF10C protein
| TRAIL R3 (Ala26 - Ala221) Accession # Q05D71 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala26
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
75-90 kDa, reducing conditions
Activity
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 15-60 ng/mL in the presence of 12 ng/mL of rhTRAIL (Catalog # 375-TEC).
The ED50 for this effect is 15-60 ng/mL in the presence of 12 ng/mL of rhTRAIL (Catalog # 375-TEC).
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 630-TR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 630-TR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TRAIL R3/TNFRSF10C
Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. In the new TNF superfamily nomenclature, TRAIL R3 is referred to as TNFRSF10C. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
References
- Sheridan, J.P. et al. (1997) Science 277:818.
- Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 3
Alternate Names
CD263, DcR1, TNFRSF10C, TRAILR3
Entrez Gene IDs
8794 (Human)
Gene Symbol
TNFRSF10C
UniProt
Additional TRAIL R3/TNFRSF10C Products
Product Documents for Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera Protein
Product Specific Notices for Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera Protein
For research use only
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