Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 633-TR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TRAILR4/TNFRSF10D protein
| Human TRAIL R4 (Ala56 - His211) Accession # Q9UBN6 |
IEGRID | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala56
Predicted Molecular Mass
44.2 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions
Activity
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 6.00-60.0 ng/mL in the presence of 20 ng/mL of rhTRAIL.
The ED50 for this effect is 6.00-60.0 ng/mL in the presence of 20 ng/mL of rhTRAIL.
Formulation, Preparation and Storage
633-TR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TRAILR4/TNFRSF10D
Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
References
- Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
- Pan, G. et al. (1998) FEBS lett 424:41.
- Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
- Degli-Esposti, M.A. et al. (1997) Immunity 7:813.
Long Name
TRAIL Receptor 4
Alternate Names
CD264, DcR2, TNFRSF10D, TRAIL R4
Entrez Gene IDs
8793 (Human)
Gene Symbol
TNFRSF10D
UniProt
Additional TRAILR4/TNFRSF10D Products
Product Documents for Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF
For research use only
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