Recombinant Human Transglutaminase 1/TGM1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7765-TG
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 1/TGM1 protein
Met2-Ala817, with an N-terminal Met and 6-His tag
Met2-Ala817, with an N-terminal Met and 6-His tag
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Inconclusive. Gly6, Ser7, Thr491, Ala571 identified. Poly His detected by Western blot.
Predicted Molecular Mass
89 kDa
SDS-PAGE
75-88 kDa, reducing conditions
Activity
Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
7765-TG
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Transglutaminase 1/TGM1
References
- Lorand, L. and R.M. Graham (2003) Nature Rev. Mol. Cell. Biol. 4:140.
- Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
- Farasat, S. et al. (2009) J. Med. Genet. 46:103.
Alternate Names
ICR2, KTG, LI1, TGase-1, TGK, TGM1
Gene Symbol
TGM1
UniProt
Additional Transglutaminase 1/TGM1 Products
Product Documents for Recombinant Human Transglutaminase 1/TGM1 Protein, CF
Product Specific Notices for Recombinant Human Transglutaminase 1/TGM1 Protein, CF
For research use only
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