Recombinant Human Transglutaminase 3/TGM3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4604-TG
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 3/TGM3 protein
Ala2-Glu693 (Gly654Arg), with an N-terminal Met and 6-His tag
Ala2-Glu693 (Gly654Arg), with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No result obtained
Predicted Molecular Mass
72 kDa
SDS-PAGE
72 kDa, reducing conditions
Activity
Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.
The specific activity is >450 pmol/min/µg, as measured under the described conditions.
The specific activity is >450 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4604-TG
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Transglutaminase 3/TGM3
Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).
References
- Kim, I.G. et al. (1993) J. Biol. Chem. 268:12682.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lorand, L. and R.M. Graham (2003) Nat. Rev. Mol. Cell Biol. 4:140.
- Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
- Sardy, M. et al. (2002) J. Exp. Med. 195:747.
Alternate Names
TG3, TgaseE, TGM3
Gene Symbol
TGM3
UniProt
Additional Transglutaminase 3/TGM3 Products
Product Documents for Recombinant Human Transglutaminase 3/TGM3 Protein, CF
Product Specific Notices for Recombinant Human Transglutaminase 3/TGM3 Protein, CF
For research use only
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